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BPC-157 stands for Body Protection Compound-157, a synthetic peptide consisting of 15 amino acids arranged in a specific sequence: Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val

Then, the samples were processed using modified filter-aided sample preparation (FASP) method (Winiewski et al., 2009)