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For antigen density experiments BSA-NIP(3), BSA-NIP(16) or BSA-NIP(80) (Norwegian Institute of Public Health) were coated at BSA concentrations of 2.5 g/mL, 0.5 g/mL and 0.1 g/mL, respectively, to obtain different surface densities with the same number of total NIP molecules

This step is catalyzed by the lipoic acid synthetase (also called lipoyl synthase), an enzyme containing iron-sulfur clusters that act as sulfur donors in the reaction (5)