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AGEs are proteins or lipids that glycate and bind to the Receptor for Advanced glycation end-products (RAGE) and mediate inflammatory pathways and reactive oxygen species (ROS) (8)

Glycemic Efficacy, Weight Effects, and Safety of Once-Weekly GLP-1 Receptor Agonists

Herrero P, Lopez B, Martin C

During the anaesthesia, animals breathed spontaneously, the pain reflex was tested by pinching the toes every 5 min, and the core temperature of the animals was monitored with a rectal probe and maintained at 37 0.5 C using a heating pad

All qRT-PCR samples were run in technical duplicates and the Ct method based on the reference value estimate from 3 housekeeping gene (GAPDH, GUSB and TBP) was used to calculate relative expression level of each gene and reaction