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Reverse transcription quantitative real time polymerase chain reaction (RT-qPCR) was performed using TaqMan Reagents (Life Technologies) with the only modification being that the reaction volume was reduced from 20 l to 15 l

This phase-specific design ensures sequential regulation across the inflammatory, proliferative, and remodeling stages of healing

In the context of long-term healthcare, DHTs help bridge persistent communication gaps between patients and healthcare providers

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