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Retroviral transfer of MRP1 and gamma-glutamyl cysteine synthetase modulates cell sensitivity to L-buthionine-S,R-sulphoximine (BSO): new rationale for the use of BSO in cancer therapy

The process is the same as GSH detection

Glutamine is imported into cancer cells via multiple transporters, including the Na + -dependent transporters, system ASC (alanine/serine/cysteine-preferring) that function as obligatory exchangers, and the Na + -coupled neutral amino acid transporters (SNATs), which belong to the SLC38 superfamily (Cha et al., 2018

Shaking introduces air bubbles, which means oxygen exposure and potential foaming

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