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Supernatants were filtered over 0.2 m filters and stored at -20C until analysis

High-dose oral B12 (1,000 to 2,000 mcg daily) can actually correct deficiency even in some people with poor absorption, because a small percentage of B12 is absorbed passively (without intrinsic factor)

After exposing the spine, the mice were fixed in a stereotaxic apparatus (ANATECH, Beijing)

In the acute phase, symptoms include euphoria, anxiety, paranoia, and psychomotor agitation, which are primarily caused by the short-term overactivation of neurotransmitter systems (Cruickshank and Dyer, 2009)

Molecular physiology of natriuretic peptide signalling