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The supernatant were removed, and the pelleted cells were resuspended in 5 mL of ACK (150 mM NH 4 Cl, 10 mM KHCO 3, 0.1 mM EDTA, pH 7.4) lysis buffer for 5 min, after which the solution was diluted with 5 mL of complete media and spun down at 300 G for 8 min at 4 o C

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