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Niacin (Vitamin B3) contributes to the maintenance of normal skin

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Together, the three components address sleep regulation from three complementary angles circadian / pineal signaling (Pinealon), delta-wave sleep induction (DSIP), and stress / anxiety modulation (Selank)

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Cells were lysed in radioimmunoprecipitation assay buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate and 0.1% SDS) and protein content was measured using a standard BCA kit (Beyotime, P0009)