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Results Validation Success: Method validated per ICH M10 guidelines for 1500 ng/mL range Stability: Demonstrated 64-day stability at both 20C and 80C Selectivity: Chromatograms free of interferences across all plasma types Matrix Effects: Assay free of matrix effects across all plasma types

discard and reconstitute fresh Not labelling the vial: Without a reconstitution date, you cannot know if the solution is within its usable window Leaving reconstituted peptide at room temperature: Degradation accelerates outside refrigeration

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Replicates, randomization and blinding In vivo studies were performed in male mice that were randomly distributed in groups matched for genotype, age, body weight and body composition (fat and lean tissue mass)

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