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While the vaccine, Heplisav-B, initially won approval for the prevention of hepatitis B in 2017, its path to the market was rocky, with two previous rejections in 2013 and 2016 for unresolved safety concerns, per Reuters

These sequences were annealed and cloned between Hin d III and Bam HI restriction sites in the p Silencer 3.0H1 vector (Ambion), downstream to the H1-RNA polymerase-3 promoter

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