Dual luciferase reporter assay Reporter plasmids of PPARa were constructed and cloned into the GV712 luciferase vector (Component sequence: CMV enhancer-MCS-SV40-puromycin)
Abcam, MA, USA), anti-FABP1 (13368S, Cell Signaling, Ma, USA and anti-ACC (3676S, cell signalling, MA, USA)
References Enlarged image of chemical structure
During mealtime, the network presented in this study driven by OB GLP-1R may contribute to a decrease in SNS activity, and thus participate in the increase of glucose-induced insulin secretion, in concert with systemic GLP-1 and the GLP-1 dependent neuronal circuitry of the brainstem and hypothalamus recruited under normal physiological circumstances
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