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coli JM109DE3 expression host the recombinant His6-AV-Ubiquitin-Semapeptide (nucleotide sequence) -pET24 plasmid vector was transformed into E.coli JM109DE3 cells by heat shock method
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When comparing the global mutagenesis profile, the effects on exendin-4 were similar to those on GLP-1, with a strong positive correlation observed for the mutagenesis data for both affinity and cAMP production, albeit that the effect on cAMP signalling was generally smaller for exendin-4 (Figures 4-5)
