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Tapia G, Fernndez V, Varela P, Cornejo P, Guerrero J, Videla LA

doi: 10.1007/s00726-005-0235-4 5 CynoberL.Le BoucherJ.VassonM

Defining a unified rationale for DHA-induced regulation of Gpx4 gene expression Our initial observations in HT22 indicated that the upregulation of Gpx4 expression occurred with a significant delay form the initial exposure to DHA

The collected samples were resuspended in a 0.1% formic acid solution (0.1% formic acid, 2% acetonitrile, and 98% Milli-Q water) and analyzed on an Eksigent C18 column (150 mm 75 m, 3 m) using a gradient mobile phase of 98% acetonitrile, 2% Milli-Q water (A) and 0.1% formic acid (B) at a flow rate of 0.3 L/min

The findings continue to be discussed and interpreted, with the interpretation of the breadth of the gene-expression effects an area where the literature has been more descriptive than mechanistically resolved