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The energy necessary for the transport of these neurotransmitters into the secretory vesicles is provided by the proton gradient generated by the vacuolar H + -ATPase (V-ATPase)

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The aqueous mobile phase (A) consisted of H2O:ACN: FA:PFHA (99.3:0.5:0.1:0.1 v/v/v/v), and the organic mobile phase (B) consisted of H2O:FA (99.9:0.1 v/v)

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