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and for Boc-His(Boc)-OH.DCHA, the coupling was performed by a HOAt/HATU/ the refined peptide solution was transformed into salts using Waters 2545 RP-HPLC system with a chromatographic column of 50 x 250 mm reverse phase C18 column and 0.1% hydrochloric acid/acetonitrile as a mobile phase

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