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The expression of specific mRNAs was assayed by fluorescence-based quantitative real-time PCR (RT-qPCR) using a Fast-SYBR PCR Master Mix (Applied Biosystems)

(Hammer Nutrition Direct, 2019)

Obesity is a complex disease, Reid says, and many people struggle with weight their whole life

Chitosan-based NPs can adhere to mucus membranes, facilitating prolonged and localized drug release, and are widely utilized in wound healing, ocular therapy, periodontal and cardiac repair, and orthopedic tissue regeneration [131]