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Eur J Heart Fail 19(12):15741585 Balestrieri G, Limonta R, Ponti E, Merlo A, Sciatti E, DIsa S et al (2024) The therapy and management of heart failure with preserved ejection fraction: new insights on treatment

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After that, cells were washed with ice-cold PBS and lysed by 50 L lysis buffer (PBS supplemented with 20 mM Tris-HCl and 1% (v/v) Triton X-100, pH 7.4)

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