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NIX, also known as BNIP3L, which shares 56% homology with BNIP3, is a member of the pro-apoptotic subfamily of the B-cell lymphoma-2 (Bcl-2) family, possessing the BH3 structural domain and capable of binding to Bcl-2 [68]

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Protein unfolding was monitored by tryptophan fluorescence (ratio 350 nm/330 nm) during temperature ramping from 25 C to 90 C

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